![]() This includes haem biosynthesis, calcium signalling, and apoptosis regulation. Mitochondria are a fundamental organelle in eukaryotic organisms, where they are responsible for a wide range of biosynthetic and metabolic processes. ![]() However, sample freezing is not always compatible with certain research interests, including mitochondrial respiration. Sample freezing has consistently proven to be effective when making assessments of genomes, transcriptomes and proteomes of a given sample. This increases the accessibility of clinically relevant samples to researchers and institutions where fresh samples might not be available. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist.Īs the field of biomedical research continues to expand throughout the 21 st century, the use of frozen tissue in research laboratories will continue to expand. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper.įunding: This work was supported by the Biotechnology and Biological Sciences Research Council (grant number BB/J014508/1), via an award to B.E. Received: SeptemAccepted: Published: July 24, 2023Ĭopyright: © 2023 Ebanks et al. PLoS ONE 18(7):Įditor: Marc Liesa, University of California Los Angeles, UNITED STATES Citation: Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer.
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